| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Townsend, Rachel A (16)
|
MRBGNW |
24.61 |
26.05 |
1.44 |
| 2 |
Dalton, Ciara I (15)
|
MRBGNW |
25.57 |
26.63 |
1.06 |
| 3 |
Ketron, Anna E (15)
|
MRBGNW |
25.30 |
26.69 |
1.39 |
| 4 |
Lee, Jamie T (17)
|
MRBGNW |
24.51 |
26.81 |
2.30 |
| 5 |
Redpath, Frances M (15)
|
MRBGNW |
26.38 |
26.86 |
0.48 |
| 6 |
Elms, Gaige L (17)
|
MRBGNW |
25.94 |
27.14 |
1.20 |
| 7 |
Shannon, Kayla (15)
|
MRGAEL |
26.15 |
27.55 |
1.40 |
| 8 |
Zhu, Catherine (15)
|
MRBGNW |
27.20 |
27.87 |
0.67 |
| 9 |
McHugh, Amanda K (16)
|
MRBGNW |
26.82 |
28.26 |
1.44 |
| 10 |
Modugno, Sabina T (15)
|
MRNYSA |
27.09 |
28.42 |
1.33 |
| 11 |
Leishman, Belle E (16)
|
MRNYSA |
28.21 |
28.86 |
0.65 |
| 12 |
Garofolo, Juliette M (16)
|
MRBGNW |
27.68 |
28.96 |
1.28 |
| 12 |
Washington, Michelle G (16)
|
MRBGNW |
28.78 |
28.96 |
0.18 |
| 14 |
Sanchez, Samantha M (15)
|
MRBGNW |
28.88 |
29.24 |
0.36 |
| 15 |
Ford, Sydney E (15)
|
MRBGNW |
28.56 |
29.44 |
0.88 |
| 16 |
Christensen, Marcella R (16)
|
MRNYSA |
28.70 |
29.49 |
0.79 |
| 17 |
Koestler, Aimee J (16)
|
MRBGNW |
31.80 |
35.04 |
3.24 |